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In Vitro Fertilization

IVF is a process of fertilization wherein an egg is combined with sperm in the laboratory followed by transfer of the developed embryo in the woman’s womb.It has brought about a revolutionary change in treating fertility problems.

Which couples require IVF ?
  • Previously failed IUI cycles
  • Blockage of fallopian tubes on both sides
  • Female partner with poor egg giving capacity
  • Severe endometriosis
  • Low sperm count
  • Couples with all normal investigations and with unknown cause of infertility
  • Couples requiring donor eggs
The IVF Procedure

  • Basic sonography,hormonal evaluation & blood investigations are done.
  • Husband’s semen analysis, culture ,basic blood investigations & hormonal evaluation are done.
  • Initial clinical examination of the couple is done.
  • Ovarian Stimulation with Fertility Medication.
  • The fertility medications used in IVF are designed to stimulate the ovaries to produce multiple mature eggs (oocytes) rather than a single egg that normally matures during the natural cycle. Retrieval of multiple eggs increases the probability of pregnancy since it means several embryos may be available for placement into the uterus.
  • Depending on the case history,age and weight of the patient,type and dosage of hormonal medications are given for stimulation.Certain adjuvant medicines are given to improve the egg quality and blood supply to the lining of the uterus.
Oocyte (Egg) Retrieval

    This procedure is planned 34-35 hours post injection given for rupture of the follicles.Retrieval of eggs or the ovum pickup is done or performed transvaginally using Sonography guidance under general anaesthesia. Patient has to come fasting minimum of 6 - 7 hours & the follicles will be punctured & fluid will be collected & seen under microscope & the eggs are picked up & transferred in the culture media for further treatment with sperms.

    After the eggs have been transferred in the culture media,they are washed in the media & then depending on the count of husband further treatment with sperms is done. Semen is washed & prepared. Depending on the count it is either IVF i.e In vitro fertilization where sperms & eggs are kept close to each other for fertilization or ICSI (i.e. Intra Cytoplasmic Sperm injection) is done where you manually pickup one sperm under microscopic vision which is a special instrument by itself called as micromanipulator and inject in the oocyte or egg. In the lab they are fertilized till Day 2,3, or Day 5 depending on how they are progressing.

Embryo Transfer

    Embryos are transferred into the uterus through a catheter. This procedure is done under short anaesthesia or without anaesthesia under sonography guidance and is painless.

    The embryos are loaded with a small amount of fluid inside the catheter, which is passed through the cervix at the time of a speculum examination. The embryos are deposited in a manner so they reach the top part of the uterus. Depending upon the quality of the embryos, one to three embryos may be transferred in one treatment cycle.

Why choose us ?

One of the first few IVF Centres pioneering the technique of slow freezing and vitrification.

Highly experienced embryologist with 20 years of experience.

More than 50% pregnancy rates with frozen embryo transfer which is comparable to fresh cycle rates.

Survival of frozen embryos post warming depends to a great extent on how the warming process is carried out which requires skilled embryologist.

Cost effective freezing packages.

ICSI (Intracytoplasmic sperm injection)

ICSI (Intra Cytoplasmic Sperm Injection) or Micromanipulation is a technique in which one egg is mechanically fertilized with single sperm.
Do we need ICSI? ICSI is an excellent advancement in the field of assisted reproduction which is required in certain situations where IVF may fail.We recommend ICSI in following conditions

  • When the male partner has an extremely low sperm count,poor sperm motility(the speed with which the sperms move),abnormal looking sperms.
  • When sperms are retrieved surgically from male testicles in patients having zero sperm count.
  • Couples with previous failed IVF cycles
  • Unexplained infertility
How is ICSI done?

The ICSI technique makes use of a powerful inverted microscope at the magnification of about 400X and micromanipulator to inject a single sperm in to the ooplasm. The skilled Embryologist with the help of fine pipette holds a single egg by applying gentle negative pressure and injects a single sperm in to the egg with the help of other micro injecting pipette.

What happens post ICSI?

The embryologist will look for signs of fertilization the next day and the embryos are further cultured in the laboratory. Depending upon the growth of embryos,good quality embryos after careful monitoring and grading are then selected by the embryologist for transfer on Day 3.Embryos can also be transferred on day 5 when they reach blastocyst stage.

Are babies born through ICSI healthy?

ICSI is just a method of assisted reproduction and it doesn’t affect the genetic make up of the embryo in any form.The process doesn’t harm the egg or result in abnormal embryo.Babies born through ICSI are absolutely normal and ICSI doesn’t have any future impact on the baby’s health.

Our centre has an almost 100% ICSI fertilization rate and it is performed by our chief embryologist, Mrs. Priti Trivedi, a pioneer in her field with more than 20 years of experience.

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